tet on 3g system  (TaKaRa)

Journal: Redox Biology

Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals

doi: 10.1016/j.redox.2026.104105

Figure Lengend Snippet: Generation and validation of club cell-specific AHR knockout mice ( Ahr ΔCC). (a) Schematic of breeding strategy to generate Ahr ΔCC mice by crossing Ahr fl/fl mice with Scgb1a1-CreER TM mice, followed by tamoxifen induction. (b) Immunofluorescence staining showing club cell marker CC10 (green), AHR (red) and DAPI (blue) in lung sections of Cre-negative Ahr fl/fl LM control (top panel) and Ahr ΔCC (bottom panel) mice. (c) Representative flow cytometry plots of lung epithelial cells gated as CD45 − CD31 − EpCAM + CC10 + cells isolated from lungs of LM (left) or Ahr ΔCC (center) mice and Fluorescence-minus-one (FMO) control for AHR staining (right). (d) Quantification of the percentage of AHR + CC10 + cells in the lungs of LM (white bar) and Ahr ΔCC (gray bar) mice. Data represent mean ± SEM, n = 3-4 mice per group. Statistical significance was determined using Student's t-test; ∗p < 0.05.

Article Snippet: Male Ahr tm3.1Bra /J mice carrying a floxed exon 2 allele of the Ahr gene (JAX stock #006203) and female B6N.129S6(Cg)- Scgb1a1 tm1(cre/ERT)Blh /J mice expressing tamoxifen-inducible Cre recombinase under the control of the club cell-specific Scgb1a1 promoter (JAX stock #016225) were obtained from Jackson Laboratory.

Techniques: Biomarker Discovery, Knock-Out, Immunofluorescence, Staining, Marker, Control, Flow Cytometry, Isolation, Fluorescence

Journal: PLOS One

Article Title: Biomaterial-associated molecular patterns (BAMPs) modulate macrophage polarization in bone grafting

doi: 10.1371/journal.pone.0345787

Figure Lengend Snippet: (A) Representative confocal microscopy images demonstrate iNOS expression in macrophages post-treatment with control PBS, LPS, IL-4, DMB at 50 µg/mL of DMSP, and the DCC group with DCSP at 50 µg/mL. Nuclei are stained with DAPI (blue), while iNOS displays a red fluorescence signal labelled with PE fluorochrome as the intracellular marker. The merged images of DAPI and iNOS-PE include a 25 µm scale bar. (B) Confocal microscopy images exhibit the immunofluorescence for the macrophage marker Arg-1. Nuclei are stained with DAPI (blue), and Arg-1 is marked by a green fluorescence signal labelled with the Alexa Fluor 488 fluorochrome as the intracellular marker. (C) and (D) represent the graphical presentation of the percentage of iNOS-positive and arginase-1-positive cells, respectively. The notation ns indicates no significance, while ****p ≤ 0.0001, **p ≤ 0.01.

Article Snippet: Cells were fixed in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) for 20 min at room temperature, then permeabilized with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS for 5 min. Macrophages were immunostained with Phycoerythrin (PE)-conjugated anti-iNOS antibody for inducible nitric oxide synthase (iNOS) expression (NBP2−22119PE, Novus).

Techniques: Confocal Microscopy, Expressing, Control, Staining, Fluorescence, Marker, Immunofluorescence